The Attune NxT Flow Cytometer Autosampler is an add-on accessory compatible with Attune NxT Flow Cytometer models designed for use with this device (Cat. Nos. A24858, A24859, A24860, A24861, A24862, A24863, A24864, A28993, A28995, A28997, A28999, A29001, A29002, A29003, A29004). The Attune NxT Autosampler enables rapid processing of multiple samples by automatically analyzing samples from 96-, 384-, and deep-well plates. An Attune NxT Autosampler Accessory Kit is included. A computer is not included with the autosampler, as it is included with the cytometer itself.
Features of the Attune NxT Autosampler include:
• Reliable—no-clog instrument design with Intelligent Probe results in higher productivity, less down time
• Broad compatibility—compatible with many different plate formats, including 96-, 384-, and deep-well plates
• Consistent data—designed to provide minimal variation regardless of sampling method (tube vs. plate) and collection rate
• Mixing by aspiration—mixing of sample by aspiration instead of shaking ensures homogeneity of the sample and maintains cell viability
• Simple to use—experiment setup has never been this simple
The Attune NxT Autosampler was designed to minimize system down time. Automated sampling devices are often plagued by clogging or probe crashes. The Intelligent Probe, with a 200-micron diameter that is larger than most probes available in the industry, minimizes instances of clogging.
To prevent probe crashes, which may require expensive probe replacement, the Attune NxT Autosampler automatically performs a probe self-calibration every month to help ensure consistent positioning of the probe within every well. The self-calibration verifies proper function of the probe, reduces variability in probe positioning over time to prevent failures during plate runs, and helps improve reliability over the life of the system. Self-calibration of the probe also allows the autosampler to be easily moved, so it can be shared between multiple instruments.
The Attune NxT Autosampler allows use of many different plate formats, including 96-, 384-, and deep-well plates, without being restricted to a single manufacturer. To maintain system flexibility, the manual sample probe is always accessible even when the loader is present, and switching between tubes and plates is as simple as a flip of a switch.
In addition, the Attune NxT Autosampler initiates automated cleaning procedures when the instrument is shutting down to help ensure proper maintenance with little user interaction.
Unlike other plate loaders that mix samples by shaking, which may not ensure homogeneity of the sample, the Attune NxT Autosampler mixes samples by aspiration, enabling uniform mixing while preserving sample viability and resulting in consistent data throughout the plate.
Simple to use
The instrument design and software were developed with customer ease-of-use in mind. The Attune NxT Autosampler has fluidic bottles with sensors that inform the user when the fluids need to be replaced. The autosampler’s indicator lights and warning systems have been designed to ensure that a user who has walked away from the system can easily see the system status at all times.
Setting up an experiment with the Attune NxT cytometric software is simple. Using a plate layout view, users can create compensation wells, define instrument settings, and identify samples. The intuitive software allows quick learning and data analysis. For example, a heat map view allows rapid screening and confirmation of samples while the instrument is acquiring data.