The iBlot® 2 Gel Transfer Device is our second-generation dry transfer device, providing the same speed and convenience as the original iBlot® device, but with many new features. The iBlot® 2 Gel Transfer Device performs western blotting transfer simply, efficiently, and reliably, within seven minutes and without the need for liquid buffers. The iBlot® 2 Gel Transfer Device is an integral part of the iBlot® 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF). The iBlot® 2 device and the transfer stacks are sold separately.
Features of the iBlot® 2 Dry Blotting System:
• Complete protein transfer in 7 minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
• A simple, user-friendly system
• Options to create new custom programs
• Built-in tutorial and application notes
• High-quality transfer stacks that are more compact than before
Using the iBlot® 2 System for Rapid, High-quality Protein Transfers
The iBlot® 2 Dry Blotting System is for dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for western detection. The iBlot® 2 system offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency. Additionally, the iBlot® 2 Dry Blotting System offers new options that allow users to create their own custom programs.
How It Works
Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers. The high density of ions in the gel matrix enables rapid protein transfer. During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen). With the iBlot® 2 Dry Blotting System, transfer time is reduced by the shortened distance between electrodes, high field strength, and high current. Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to our unique de-bubbling design that promotes even and complete transfer.
iBlot® 2 Transfers Save You Time
With the iBlot® 2 system, there is no need to prepare buffers, pre-treat your gel, or clean up after blotting. The total preparation and run time is normally less than 10 minutes per blot. Compared with conventional western transfer techniques, iBlot® 2 transfers can save you hours.