The EVOS® FL Auto Imaging System is a fully-automated, digital, inverted multi-channel fluorescence and transmitted light imaging system with outstanding workflow efficiency. Designed to meet demanding requirements over a broad range of applications, the EVOS® FL Auto system supports high-resolution mosaic tiling, multi-position well scanning, cell counting with thresholding, and time-lapse studies. The intuitive interface, proprietary light cubes, dual cameras, precision automated stage and parfocal optical system enables you to produce publication quality images in seconds.
The EVOS® FL Auto Imaging System offers you these important advantages:
• Exceptional ease-of-use with a fully automated and motorized X/Y scanning stage, autofocus and flat-focus Z-stack
• Advanced software featuring high-resolution mosaic tiling, multi-position well scanning, object counting, and time-lapse
• Outstanding versatility with a 5-position objective turret, 4-color fluorescence, plus color and B/W cameras
• 22" touch-screen LCD display, networking capability, and DVI output
Fully Automated Imaging System
The EVOS® FL Auto system combines and integrates precision components with a unique design functionality that enables high-quality automated fluorescence imaging with superior work-flow efficiency. Full automation of the precision X/Y-stage movement; automated changes of up to 4 LED fluorescence light cubes and 5 parfocal objectives, focus, and exposure; and dual camera switching makes the EVOS® FL Auto system an exceptional platform for a variety of demanding imaging applications. Program the EVOS® FL Auto system to run acquisition routines, 8-point time lapse experiments, and tile-stitch scans in nearly any vessel through the sensitive touch-screen display.
Versatile and Highly Configurable
With a 5-position objective turret and 4 fluorescence light cube positions, the optical system can be easily configured to meet your needs. Choose from our full range of high-quality objectives from 2x to 100x magnification, and over a dozen LED light cubes. The EVOS® FL Auto system automatically adjusts the lighting settings to match the objective magnification. Dual cameras with a Sony ICX445 CCD monochrome and a high sensitivity CMOS color digital camera enable seamless imaging of both fluorescent and colorimetrically stained samples. Interchangeable stage plates and vessel holders accommodate most vessel types and sizes, including multi-well plates, slides, culture flasks, and Petri dishes, and afford precise control and sample alignment by the automated stage.
Smart LED Illumination Technology
All EVOS® fluorescence imaging systems utilize our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light-path that delivers superior fluorophore excitation. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching. Hard-coated filters give sharper edges and significantly higher transmission efficiencies than traditional soft-coated filters.
Fully Integrated Environmental Control System
When used with the EVOS® Onstage Incubator (available separately), the EVOS® FL Auto Imaging System affords a complete solution for environmental control during live cell imaging studies. Specifically designed for use with the EVOS® FL Auto system, the incubator consists of an environmental control unit and an environmental chamber that is precision-tooled for the automated X-Y stage. A small, separate control unit supplies the power, gas (air or air-CO2 premix, CO2, and N2 for O2 displacement in hypoxia experiments), and control of humidity and temperature. Unlike many other systems that require separate software modules or control units, the EVOS® Onstage Incubator is operated through the EVOS® FL Auto interface. Following a simple installation, the incubator is automatically recognized by the imaging software and is ready for use.
The EVOS® FL Auto system is powered by an associated PC that controls the fully automated imaging system, including creating, saving, and running routines and experiments, as well as image editing and manipulation. Whether using the step-by-step guide or manually defining acquisition parameters, the EVOS® FL Auto system is remarkably easy to use and run; the high-resolution 22" LCD touch screen display enables "finger swipes" to expand or zoom in on images, and moving between microwell plate wells is done by a single touch. Versatile and powerful software features include mosaic image stitching, automated multi-position acquisition in single image or time-lapse mode, and automated cell counting with watershed algorithm.
Easy to Use and Reliable
The EVOS® FL Auto Imaging System is powerful, yet offers the simplicity of plug-and-play. Like other EVOS® systems, it requires no warm-up or cool-down periods. The LED light source offers exceptional stability and durability—so you can turn the unit on and off whenever you need to image a sample. The environmentally safe, mercury-free LED bulbs are rated for >50,000 hours (~17 years) of life, compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. The long life and low energy consumption translate into significantly lower operating costs compared to instruments with conventional light sources.
The EVOS® Imaging Systems are built from the ground up to maximize performance and optimize workflow. You will be astonished at how easy it is to operate and amazed at how extraordinary your images look on-screen.
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Auto exposure: adjustment for optimal exposure times
Autofocus: can be set up in several different modes to optimize speed and accuracy
Automated optical system: automated switching of parfocal objectives, LED light cubes, and monochrome/color cameras
Counting function: automated cell counting with watershed algorithm for easy GFP expression monitoring, live/dead assessment, and total cell count using NucBlue® Live
Custom pseudocolor: choose display color for each cube (monochrome camera only)
Freehand annotation tool: highlight areas/cells of interest using the touch-screen controls or mouse to draw freehand shapes
Image editing: Counting, measurements, annotation tools, and image review; automatic overlay, select/deselect channels, brightness/contrast adjustments, and saving to USB or network
Image stitching: capture multiple images with overlapping fields and use mosaic tiling to stitch a high-resolution image of a large area
Image Review: bring in images for editing or object counting
Manual exploration: define parameters optimal for your experiments
Measure and annotate: add annotations and scale bar
Quick Image Wizard: step-by-step guide to capture publication-quality multicolor images
Reduced photobleaching: variable illumination control of digital LED light source and camera signal amplification helps dramatically reduce photobleaching
ROI definition: define regions of interest (ROI) for scan routines
Routines: create, save, and run repeat routines and experiments
Scalebar: a scalebar can be added to a captured image via the System Basics tab
Scan review: zoom in/out and pan, export the entire scan or only regions of interest
Time lapse: use up to 384 beacons to seamlessly capture time-lapse movies on slides or microplates
Z-Stack flat focus: collect a series of images; extract the most "in focus" pixels from each to create a single, focused image even from thick samples
System & Hardware
Optics: infinity-corrected optical system, RMS-threaded objectives with 45 mm parfocal distance
Illumination: adjustable-intensity LED (>50,000-hour life per light cube)
Light cubes (not included): broad selection of patented standard and specialty light cubes
Commonly used light cubes include: DAPI (Ex 360 nm/Em 447 nm), GFP (Ex 470 nm/Em 525 nm), RFP (Ex 530 nm/Em 593 nm), Texas Red® (Ex 585 nm/Em 624), Cy5 (Ex 628 nm/Em 692 nm)
Contrast methods: fluorescence and transmitted light (brightfield & phase contrast)
Objective turret: 5-position, front-mounted control
Objectives (not included): wide selection of high-quality fluorite LWD and coverslip-corrected objectives
Condenser: 60 mm long working distance condenser with automatic objective detection, 4-position aperture wheel for use with phase contrast or configurable inserts, maintains proper agreement of any phase contrast objectives (condenser to objectives)
Stage: motorized, encoded X/Y scanning stage; travel range: 115mm x 78mm with sub-micron resolution; drop-in inserts to receive vessel holders and lock down mechanism to fix sample in place during long scans
Focus mechanism: automated focus mechanism with sub-micron resolution
LCD display: 22" high-resolution touch screen color monitor (also fully controllable via mouse), 1920 x 1080 pixel resolution
Camera: dual monochrome and color cameras; Sony ICX445 monochrome CCD, 1/3" 1280 x 960 pixels, 1.3 megapixels; high sensitivity color CMOS digital camera, 1360 x 1024 pixels, 3.1 megapixels.
Computer: external PC with 16 GB RAM running Windows® 7 Pro, designed to operate with touch-screen monitor and instrument
Captured images: 16-bit monochrome TIFF or PNG (12-bit dynamic range); 24-bit color TIFF, PNG JPG, BMP; time-lapse AVI
Output ports: multiple USB Ports, 1 display output with DVI adaptor (supports direct output to USB and networked storage)
Networking capability: connection through Windows/SMB network via an ethernet cable connection
Power supply: AC adaptor with country-specific power cords
Dimensions: height = 322/12.7, width = 343/13.5, depth = 472/18.6 (inches/mm)